President Trump issued an executive order rescinding Executive Order (EO) 14021, Guaranteeing an Educational Environment Free From Discrimination on the Basis of Sex, Including Sexual Orientation or Gender Identity, signed by President Biden in March 2021. EO 14021 explicitly stated that all students should be guaranteed an educational environment free from discrimination on the basis of sex, including discrimination in the form of sexual harassment, sexual violence, and on the basis of sexual orientation or gender identity. EO 14021 included actions for the Secretary of Education on enforcement against discrimination and sexual harassment against LGBTQ+ students.

The rescissions of EO 14021 appears in two executive orders: Initial Rescissions of Harmful Executive Orders and Actions, and Section 7(b) of Defending women from gender ideology extremism and restoring biological truth to the federal government.